Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00006938-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006938-M01, RRID:AB_2255600
- Product name
- Mouse Monoclonal TCF-12/HTF4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TCF12 - transcription factor 12 (HTF4, helix-loop-helix transcription factors 4)
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of TCF12 over-expressed 293 cell line, cotransfected with TCF12 Validated Chimera RNAi ( Cat # H00006938-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TCF12 monoclonal antibody (M01), clone 2E9 (Cat # H00006938-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of TCF12 expression in transfected 293T cell line by TCF12 monoclonal antibody (M01), clone 2E9.Lane 1: TCF12 transfected lysate(75.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 monoclonal antibody (M01), clone 2E9 Analysis of TCF12 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 ( Cat# H00006938-M01 ) on HeLa cell . [antibody concentration 10 ug/ml]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of monoclonal antibody to TCF12 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.