PA5-21039

antibody from Invitrogen Antibodies

Targeting: TCF12 bHLHb20, HEB, HsT17266, HTF4, p64

Provider product page for PA5-21039

Western blot

Immunocytochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [3]

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Product number: PA5-21039 - Provider product page
Provider: Invitrogen Antibodies
Product name: TCF12 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control is Hela cell lysate. PA5-21039 can be used with blocking peptide PEP-1153.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

TCF12 protein structure - PA5-21039 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Transcription matrix for selected WASp enriched genes. a Bioinformatic association of WASp ChIP-seq peaks from our datasets with activating (A) and/or repressive (R) epigenetic regulatory marks, RNA Polymerase II and TFs peaks and lamin B1 marks in thymocytes (T) and spleen CD4 + cells (S). b Association of WASp ChIP-seq peaks in thymocytes of Tcf12 , Vav2 , and Wasf2 with RNA Polymerase II ChIP-Seq signal by TFBS ENCODE/LICR resource at UCSC genome browser

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 The activation status of nuclear WASp affects TCF1-mediated Tcf12 expression. a TCF12 in NE and CE from WT, WASp KO, WASp I296T , and WASp L272P thymocytes. These data were repeated twice with independent biological replicates. Detection of GAPDH was used as a sample loading control for total lysate and for cytosolic extract. Detection of histone H3 was used as a sample loading control for total lysate and for nuclear extract. The quantification of nuclear and cytosolic TCF12 in the western blot is shown in the right panel . b Consensus DNA motif determined by WASp ChIP-seq data analysis and the canonical TCF1 DNA-binding site. c Proximity of WASp ChIP-seq gene peak positions and TCF1-binding sites in Tcf12 intron 3. d TCF1 in NE and CE from WT, WASp KO, WASp I296T , and WASp L272P thymocytes. These data were repeated twice with independent biological replicates. Detection of GAPDH was used as a sample loading control for total lysate and for cytosolic extract. Detection of histone H3 was used as a sample loading control for total lysate and for nuclear extract. The quantification of nuclear and cytosolic TCF1 in the western blot is shown in the right panel. e Co-IP of WASp and TCF1 from thymocyte nuclear extracts. These data were repeated three times with independent biological replicates. Purified mouse IgG 2A was used as the isotype Ab-negative control IP. Heavy chain of IgG (IgGhc) is observed in eluted IP samples. Additional file 1 : Figure S6 and S7 and Additiona

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 Variation of distances from WASp ChIP-Seq gene peaks to nearest exon(s). Selected examples of genes with ( a ) shorter distance range (0.5-5 kb) and ( b ) longer distance range (5-50 kb) between WASp ChIP-seq gene peak position and nearest exon. Additional file 1 : Figure S4