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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF7197 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Lipoprotein Lipase/LPL Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Lipoprotein Lipase/LPL in Western blots. Detects human Lipoprotein Lipase/LPL in direct ELISAs and less than 1% cross-reactivity with recombinant human (rh) LIPG, rhLIPI, and rhPNLIPRP1 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references FiremasterĀ® 550 and its components isopropylated triphenyl phosphate and triphenyl phosphate enhance adipogenesis and transcriptional activity of peroxisome proliferator activated receptor (PparĪ³) on the adipocyte protein 2 (aP2) promoter.
Tung EWY, Ahmed S, Peshdary V, Atlas E
PloS one 2017;12(4):e0175855
PloS one 2017;12(4):e0175855
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Lipoprotein Lipase/LPL by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipoprotein Lipase/LPL at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Lipoprotein Lipase/LPL by Western Blot. Detection of Human Lipoprotein Lipase/LPL by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Lipoprotein Lipase/LPL in Human Heart. Lipoprotein Lipase/LPL was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human/Mouse Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
