Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [34]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3075 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal SOX9 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) SOX10 is observed, and less than 15% rhSOX11, rhSOX12, and rhSOX15 is observed, and less than 5% cross-reactivity with rhSOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX8, rhSOX13, rhSOX17, rhSOX18, rhSOX21, and rhSOX30 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Lack of whey acidic protein (WAP) four-disulfide core domain protease inhibitor 2 (WFDC2) causes neonatal death from respiratory failure in mice.
Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue.
Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation.
3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling.
Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.
Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusion.
Restorative effects of human neural stem cell grafts on the primate spinal cord.
Glutamate transporter Slc1a3 mediates inter-niche stem cell activation during skin growth.
Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity.
Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells.
In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus.
Does human endometrial LGR5 gene expression suggest the existence of another hormonally regulated epithelial stem cell niche?
Meis1 Coordinates Cerebellar Granule Cell Development by Regulating Pax6 Transcription, BMP Signaling and Atoh1 Degradation.
Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1.
Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.
Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors.
An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos.
YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis.
Fate mapping neurons and glia derived from Dbx1-expressing progenitors in mouse preBötzinger complex.
SOX9 Is an Astrocyte-Specific Nuclear Marker in the Adult Brain Outside the Neurogenic Regions.
Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate.
The development and plasticity of alveolar type 1 cells.
Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
Two nested developmental waves demarcate a compartment boundary in the mouse lung.
Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.
In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column.
Identification of SOX3 as an XX male sex reversal gene in mice and humans.
The vanishing testis: a histomorphologic and clinical assessment.
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines.
A novel approach for the derivation of putative primordial germ cells and sertoli cells from human embryonic stem cells.
FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD).
Nakajima K, Ono M, Radović U, Dizdarević S, Tomizawa SI, Kuroha K, Nagamatsu G, Hoshi I, Matsunaga R, Shirakawa T, Kurosawa T, Miyazaki Y, Seki M, Suzuki Y, Koseki H, Nakamura M, Suda T, Ohbo K
Disease models & mechanisms 2019 Nov 12;12(11)
Disease models & mechanisms 2019 Nov 12;12(11)
Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue.
Tysoe OC, Justin AW, Brevini T, Chen SE, Mahbubani KT, Frank AK, Zedira H, Melum E, Saeb-Parsy K, Markaki AE, Vallier L, Sampaziotis F
Nature protocols 2019 Jun;14(6):1884-1925
Nature protocols 2019 Jun;14(6):1884-1925
Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation.
Romeo SG, Alawi KM, Rodrigues J, Singh A, Kusumbe AP, Ramasamy SK
Nature cell biology 2019 Apr;21(4):430-441
Nature cell biology 2019 Apr;21(4):430-441
3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling.
Zhang Y, Yang Y, Jiang M, Huang SX, Zhang W, Al Alam D, Danopoulos S, Mori M, Chen YW, Balasubramanian R, Chuva de Sousa Lopes SM, Serra C, Bialecka M, Kim E, Lin S, Toste de Carvalho ALR, Riccio PN, Cardoso WV, Zhang X, Snoeck HW, Que J
Cell stem cell 2018 Oct 4;23(4):516-529.e5
Cell stem cell 2018 Oct 4;23(4):516-529.e5
Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.
Jones JR, Kong L, Hanna MG 4th, Hoffman B, Krencik R, Bradley R, Hagemann T, Choi J, Doers M, Dubovis M, Sherafat MA, Bhattacharyya A, Kendziorski C, Audhya A, Messing A, Zhang SC
Cell reports 2018 Oct 23;25(4):947-958.e4
Cell reports 2018 Oct 23;25(4):947-958.e4
Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusion.
Castle MJ, Cheng Y, Asokan A, Tuszynski MH
Science advances 2018 Nov;4(11):eaau9859
Science advances 2018 Nov;4(11):eaau9859
Restorative effects of human neural stem cell grafts on the primate spinal cord.
Rosenzweig ES, Brock JH, Lu P, Kumamaru H, Salegio EA, Kadoya K, Weber JL, Liang JJ, Moseanko R, Hawbecker S, Huie JR, Havton LA, Nout-Lomas YS, Ferguson AR, Beattie MS, Bresnahan JC, Tuszynski MH
Nature medicine 2018 May;24(4):484-490
Nature medicine 2018 May;24(4):484-490
Glutamate transporter Slc1a3 mediates inter-niche stem cell activation during skin growth.
Reichenbach B, Classon J, Aida T, Tanaka K, Genander M, Göritz C
The EMBO journal 2018 May 2;37(9)
The EMBO journal 2018 May 2;37(9)
Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity.
Tata PR, Chow RD, Saladi SV, Tata A, Konkimalla A, Bara A, Montoro D, Hariri LP, Shih AR, Mino-Kenudson M, Mou H, Kimura S, Ellisen LW, Rajagopal J
Developmental cell 2018 Mar 26;44(6):679-693.e5
Developmental cell 2018 Mar 26;44(6):679-693.e5
Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells.
Zhang M, Ngo J, Pirozzi F, Sun YP, Wynshaw-Boris A
Stem cell research & therapy 2018 Mar 15;9(1):67
Stem cell research & therapy 2018 Mar 15;9(1):67
In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus.
Rosin JM, Kurrasch DM
Journal of neuroinflammation 2018 Jun 12;15(1):181
Journal of neuroinflammation 2018 Jun 12;15(1):181
Does human endometrial LGR5 gene expression suggest the existence of another hormonally regulated epithelial stem cell niche?
Tempest N, Baker AM, Wright NA, Hapangama DK
Human reproduction (Oxford, England) 2018 Jun 1;33(6):1052-1062
Human reproduction (Oxford, England) 2018 Jun 1;33(6):1052-1062
Meis1 Coordinates Cerebellar Granule Cell Development by Regulating Pax6 Transcription, BMP Signaling and Atoh1 Degradation.
Owa T, Taya S, Miyashita S, Yamashita M, Adachi T, Yamada K, Yokoyama M, Aida S, Nishioka T, Inoue YU, Goitsuka R, Nakamura T, Inoue T, Kaibuchi K, Hoshino M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jan 31;38(5):1277-1294
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jan 31;38(5):1277-1294
Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1.
Memon B, Karam M, Al-Khawaga S, Abdelalim EM
Stem cell research & therapy 2018 Jan 23;9(1):15
Stem cell research & therapy 2018 Jan 23;9(1):15
Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.
Lewis PL, Su J, Yan M, Meng F, Glaser SS, Alpini GD, Green RM, Sosa-Pineda B, Shah RN
Scientific reports 2018 Aug 15;8(1):12220
Scientific reports 2018 Aug 15;8(1):12220
Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors.
Aigha II, Memon B, Elsayed AK, Abdelalim EM
Stem cell research & therapy 2018 Apr 3;9(1):83
Stem cell research & therapy 2018 Apr 3;9(1):83
An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos.
Lee WJ, Chatterjee S, Yap SP, Lim SL, Xing X, Kraus P, Sun W, Hu X, Sivakamasundari V, Chan HY, Kolatkar PR, Prabhakar S, Lufkin T
BioMed research international 2017;2017:8932583
BioMed research international 2017;2017:8932583
YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis.
Lin C, Yao E, Zhang K, Jiang X, Croll S, Thompson-Peer K, Chuang PT
eLife 2017 Mar 21;6
eLife 2017 Mar 21;6
Fate mapping neurons and glia derived from Dbx1-expressing progenitors in mouse preBötzinger complex.
Kottick A, Martin CA, Del Negro CA
Physiological reports 2017 Jun;5(11)
Physiological reports 2017 Jun;5(11)
SOX9 Is an Astrocyte-Specific Nuclear Marker in the Adult Brain Outside the Neurogenic Regions.
Sun W, Cornwell A, Li J, Peng S, Osorio MJ, Aalling N, Wang S, Benraiss A, Lou N, Goldman SA, Nedergaard M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Apr 26;37(17):4493-4507
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Apr 26;37(17):4493-4507
Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate.
Laresgoiti U, Nikolić MZ, Rao C, Brady JL, Richardson RV, Batchen EJ, Chapman KE, Rawlins EL
Development (Cambridge, England) 2016 Oct 15;143(20):3686-3699
Development (Cambridge, England) 2016 Oct 15;143(20):3686-3699
The development and plasticity of alveolar type 1 cells.
Yang J, Hernandez BJ, Martinez Alanis D, Narvaez del Pilar O, Vila-Ellis L, Akiyama H, Evans SE, Ostrin EJ, Chen J
Development (Cambridge, England) 2016 Jan 1;143(1):54-65
Development (Cambridge, England) 2016 Jan 1;143(1):54-65
Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
Zhang X, Westerhoff M, Hart J
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2015 May;28(5):654-61
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2015 May;28(5):654-61
Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear CE, Post M
Stem cell reports 2015 Mar 10;4(3):419-30
Stem cell reports 2015 Mar 10;4(3):419-30
Two nested developmental waves demarcate a compartment boundary in the mouse lung.
Alanis DM, Chang DR, Akiyama H, Krasnow MA, Chen J
Nature communications 2014 May 29;5:3923
Nature communications 2014 May 29;5:3923
Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
Ramasamy SK, Kusumbe AP, Wang L, Adams RH
Nature 2014 Mar 20;507(7492):376-380
Nature 2014 Mar 20;507(7492):376-380
The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.
Mahoney JE, Mori M, Szymaniak AD, Varelas X, Cardoso WV
Developmental cell 2014 Jul 28;30(2):137-50
Developmental cell 2014 Jul 28;30(2):137-50
In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column.
Chatterjee S, Sivakamasundari V, Yap SP, Kraus P, Kumar V, Xing X, Lim SL, Sng J, Prabhakar S, Lufkin T
BMC genomics 2014 Dec 5;15:1072
BMC genomics 2014 Dec 5;15:1072
Identification of SOX3 as an XX male sex reversal gene in mice and humans.
Sutton E, Hughes J, White S, Sekido R, Tan J, Arboleda V, Rogers N, Knower K, Rowley L, Eyre H, Rizzoti K, McAninch D, Goncalves J, Slee J, Turbitt E, Bruno D, Bengtsson H, Harley V, Vilain E, Sinclair A, Lovell-Badge R, Thomas P
The Journal of clinical investigation 2011 Jan;121(1):328-41
The Journal of clinical investigation 2011 Jan;121(1):328-41
The vanishing testis: a histomorphologic and clinical assessment.
Antic T, Hyjek EM, Taxy JB
American journal of clinical pathology 2011 Dec;136(6):872-80
American journal of clinical pathology 2011 Dec;136(6):872-80
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Cavallo C, Desando G, Facchini A, Grigolo B
Journal of biomedical materials research. Part A 2010 Apr;93(1):86-95
Journal of biomedical materials research. Part A 2010 Apr;93(1):86-95
Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines.
Swartling FJ, Ferletta M, Kastemar M, Weiss WA, Westermark B
Oncogene 2009 Sep 3;28(35):3121-31
Oncogene 2009 Sep 3;28(35):3121-31
A novel approach for the derivation of putative primordial germ cells and sertoli cells from human embryonic stem cells.
Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
Stem cells (Dayton, Ohio) 2009 Jan;27(1):68-77
Stem cells (Dayton, Ohio) 2009 Jan;27(1):68-77
FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD).
Hersmus R, Kalfa N, de Leeuw B, Stoop H, Oosterhuis JW, de Krijger R, Wolffenbuttel KP, Drop SL, Veitia RA, Fellous M, Jaubert F, Looijenga LH
The Journal of pathology 2008 May;215(1):31-8
The Journal of pathology 2008 May;215(1):31-8
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SOX9 by Western Blot. Detection of Human SOX9 by Western Blot.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SOX9 by Simple WesternTM. Detection of Human SOX9 by Simple WesternTM.
