Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-36878 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SOX9 (Ser181) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 56 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SOX9 (Ser181) in Lane 1: HeLa lysate treated with PMA (100 nM,30min), Lane 2: mouse brain tissue lysate, Lane 3: rat liver tissue lysate. Samples were incubated with Phospho-SOX9 (Ser181) polyclonal antibody (Product # PA5-36878) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SOX9 pSer181 using Phospho-SOX9 pSer181 polyclonal antibody (Product # PA5-36878) at a dilution of 1:500. Lane 1: Hela lysate treated with PMA (100nM, 30min), Lane 2: Mouse brain tissue lysate, Lane 3: Rat liver tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SOX9 (Ser181) in Lane 1: HeLa lysate treated with PMA (100 nM,30min), Lane 2: mouse brain tissue lysate, Lane 3: rat liver tissue lysate. Samples were incubated with Phospho-SOX9 (Ser181) polyclonal antibody (Product # PA5-36878) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-SOX9 (Ser181) in paraffin-embedded human tonsil carcinoma tissue (nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-SOX9 (Ser181) polyclonal antibody (Product # PA5-36878) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-SOX9 (Ser181) in paraffin-embedded human colon carcinoma tissue (nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-SOX9 (Ser181) polyclonal antibody (Product # PA5-36878) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.