Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [11]
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Validation data
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- Product number
- NBP2-52943 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal SOX9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human SOX9 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
SQRTHIKTEQLSPSH
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX9 Antibody (CL0639) [NBP2-52943] - 251 Cells transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-SOX9 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX9 Antibody (CL0639) [NBP2-52943] - Lane 1: Marker [kDa], Lane 2:Human cell line U-251 MG
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of human testis shows strong nuclear immunoreactivity in a subset of cells in seminiferous tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of human prostate shows strong nuclear immunoreactivity in glandular epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of human ventricular cancer shows strong nuclear immunoreactivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of human colorectal cancer shows moderate nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of human breast cancer shows strong nuclear immunoreactivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of human stomach shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of human lung cancer shows nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of human liver shows absence of immunoreactivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing cartilage.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of mouse embryo E11 shows nuclear positivity in the developing neural tube.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SOX9 Antibody (CL0639) [NBP2-52943] - Staining of mouse embryo E14 shows nuclear immunoreactivity in the developing eye.