Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 53-9765-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX9 Monoclonal Antibody (GMPR9), Alexa Fluor™ 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This GMPR9 monoclonal antibody reacts with human and mouse Sox9, a member of the Sry HMG-box (Sox) gene family of transcription factors. Like Sry, Sox9 contains a high mobility group (HMG-box) DNA-binding domain. The Sox9 DNA-binding domain recognizes the sequence AGAACAATGG, and when bound to co-factors, functions to bend the DNA. This structural change is thought to be important for chromatin remodeling and the initiation of more broad transcriptional regulation of multiple genes. Sox9 is expressed in multiple tissue types during development and its activation of downstream targets is determined by interaction with tissue-specific co-factors. During development, Sox9 expression is important for cartilage formation (mesenchymal to chondrocyte), sex determination (testes formation), neural crest formation and retinal development. Sox9 plays a role in the differentiation of multiple cell types including glial, hair, cardiac, melanocytes, pancreatic, prostate, and intestinal epithelium. Sox9 regulates expression of the following family member genes sox5, sox6, and sox10, in addition to collagen type 2 alpha, collagen 9A1, CEACAM1, and snail2. Sox9 dysregulation is involved in craniofacial defects, cleft palate, sex reversal, and some types of cancer (melanoma, lung adenocarcinoma, and breast cancer). Sox9 has also been shown to be expressed in adult stem cells in the liver, pancreas and intestine. Applications Reported: This GMPR9 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This GMPR9 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- GMPR9
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry of fixed and permeabilized HepG2 cells using 10 µg/mL of Mouse IgG1 K Isotype Control Alexa Fluor® 488 (green, left) or 10 µg/mL of Anti-Human/Mouse Sox9 Alexa Fluor® 488 (green, right). Nuclei are stained with DAPI (blue), colocalization of signal appears aqua.
- Conjugate
- Green dye