Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-23383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The amino acid sequence used as immunogen for the ZBTB2 antibody is 100% homologous in monkey, opossum and horse, and 93% homologous in cow and chimpanzee.
- Reactivity
- Human, Mouse, Rat, Canine, Feline, Goat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells.
Notch Signaling Regulates MMP-13 Expression via Runx2 in Chondrocytes.
Xu Y, Zhang T, Chen Y, Shi Q, Li M, Qin T, Hu J, Lu H, Liu J, Chen C
Stem cells international 2020;2020:8894449
Stem cells international 2020;2020:8894449
Notch Signaling Regulates MMP-13 Expression via Runx2 in Chondrocytes.
Xiao D, Bi R, Liu X, Mei J, Jiang N, Zhu S
Scientific reports 2019 Oct 30;9(1):15596
Scientific reports 2019 Oct 30;9(1):15596
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SOX9 in formalin-fixed, paraffin-embedded mouse testis tissue. Samples were incubated in SOX9 polyclonal antibody (Product # PA5-23383) using a dilution of 5 µg/mL. Isotype control (top left) and SOX9 antibody (bottom left, right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Chondrogenic differentiation of urine-derived cells in vitro. (a) Chondrogenic gene (Sox9, Col2a1, Acan) expression compared between the chondrogenic medium and its respective basal cultures. * p < 0.05, ** p < 0.01. Sox9, sex-determining region Y-box 9; Col2a1, collagen type II; Acan, Aggrecan. (b) The Sox9 protein expression of the urine-derived cells after 7 days of chondrogenic induction. Scale bars = 15 mu m. (c) Alcian blue staining of cells after 21 days in chondrogenic or basal medium. Glycosaminoglycan deposition around the cells was seen in the chondrogenic induced medium. Scale bars = 20 mu m.
