H00027141-M01

antibody from Abnova Corporation

Targeting: CIDEB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00027141-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00027141-M01, RRID:AB_565589

Product name

CIDEB monoclonal antibody (M01), clone 2A10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CIDEB.

Antigen sequence

YLSALNPSDLLRSVSNISSEFGRRVWTSAPPPQRP
FRVCDHKRTIRKGLTAATRQELLAKALETLLLNGV
LTLVLEEDGTAVDSEDFFQLLEDDTCLMVLQSGQS
WSP


Isotype

IgG

Antibody clone number

2A10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CIDEB monoclonal antibody (M01), clone 2A10 Western Blot analysis of CIDEB expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CIDEB expression in transfected 293T cell line by CIDEB monoclonal antibody (M01), clone 2A10.Lane 1: CIDEB transfected lysate(24.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CIDEB is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to CIDEB on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol