MA1-16512

antibody from Invitrogen Antibodies

Targeting: ARNT bHLHe2, HIF-1beta

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Gel shift (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-16512

Provider

Invitrogen Antibodies

Product name

HIF-1 beta Monoclonal Antibody (H1beta234)

Antibody type

Monoclonal

Antigen

Other

Description

Suggested positive control: antigen standard for ARNT (transient overexpression lysate).

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

H1beta234

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

HIF-1alpha-targeted pathways are activated by heat acclimation and contribute to acclimation-ischemic cross-tolerance in the heart.

Maloyan A, Eli-Berchoer L, Semenza GL, Gerstenblith G, Stern MD, Horowitz M
Physiological genomics 2005 Sep 21;23(1):79-88

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Identification of functional hypoxia response elements in the promoter region of the DEC1 and DEC2 genes.

Miyazaki K, Kawamoto T, Tanimoto K, Nishiyama M, Honda H, Kato Y
The Journal of biological chemistry 2002 Dec 6;277(49):47014-21

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HIF-1 beta in HeLa cells fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. Samples were incubated in HIF-1 beta monoclonal antibody (Product # MA1-16512) using a dilution of 5 µg/mL overnight at 4 °C followed by anti-mouse DyLight 488 (Green) with a dilution of 1:500. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.