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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-52518 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V1B2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: MALRAMRGIV NGAAPELPVP TGGPAVGARE QALAVSRNYL SQPRLTYKTV SGVNGPLVIL DHVKFPRYAE IVHLTLPDGT KRSGQVLEVS GSKAVVQVFE Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.13 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice.
Tolani B, Celli A, Yao Y, Tan YZ, Fetter R, Liem CR, de Smith AJ, Vasanthakumar T, Bisignano P, Cotton AD, Seiple IB, Rubinstein JL, Jost M, Weissman JS
Nature biotechnology 2022 Dec;40(12):1834-1844
Nature biotechnology 2022 Dec;40(12):1834-1844
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of V-ATPase B2 in human cell line U-2 OS shows positivity in vesicles. Samples were probed using a V-ATPase B2 Polyclonal Antibody (Product # PA5-52518).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of ATP6V1B2 in human cell line U-2 OS using ATP6V1B2 Polyclonal Antibody (Product # PA5-52518). Staining shows localization to vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ATP6V1B2 in human pancreas using ATP6V1B2 Polyclonal Antibody (Product # PA5-52518) shows strong cytoplasmic positivity in exocrine glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ATP6V1B2 in human cervix, uterine using ATP6V1B2 Polyclonal Antibody (Product # PA5-52518) shows no cytoplasmic positivity in squamous epithelial cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ATP6V1B2 in human kidney using ATP6V1B2 Polyclonal Antibody (Product # PA5-52518) shows strong cytoplasmic positivity in cells in proximal tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATP6V1B2 in human cerebral cortex and skeletal muscle tissues using ATP6V1B2 Polyclonal Antibody (Product # PA5-52518). Corresponding ATP6V1B2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATP6V1B2 in human skeletal muscle using ATP6V1B2 Polyclonal Antibody (Product # PA5-52518) shows low expression as expected.
