Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-52518 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V1B2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MALRAMRGIV NGAAPELPVP TGGPAVGARE QALAVSRNYL SQPRLTYKTV SGVNGPLVIL DHVKFPRYAE IVHLTLPDGT KRSGQVLEVS GSKAVVQVFE Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.13 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice.
Tolani B, Celli A, Yao Y, Tan YZ, Fetter R, Liem CR, de Smith AJ, Vasanthakumar T, Bisignano P, Cotton AD, Seiple IB, Rubinstein JL, Jost M, Weissman JS
Nature biotechnology 2022 Dec;40(12):1834-1844
Nature biotechnology 2022 Dec;40(12):1834-1844
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of V-ATPase B2 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a V-ATPase B2 Polyclonal Antibody (Product # PA5-52518).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP6V1B2 in mouse cell line NIH-3T3 and rat cell line NBT-II using a ATP6V1B2 Polyclonal Antibody (Product # PA5-52518).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP6V1B2 in human cell lines SK-MEL-30 and MCF-7 using a ATP6V1B2 Polyclonal Antibody (Product # PA5-52518). Corresponding ATP6V1B2 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of V-ATPase B2 in human cell line U-2 OS shows positivity in vesicles. Samples were probed using a V-ATPase B2 Polyclonal Antibody (Product # PA5-52518).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of V-ATPase B2 in human cerebral cortex tissue shows strong positivity in neurons and neuropil. Samples were probed using a V-ATPase B2 Polyclonal Antibody (Product # PA5-52518).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATP6V1B2 in human cerebral cortex and skeletal muscle tissues using ATP6V1B2 Polyclonal Antibody (Product # PA5-52518). Corresponding ATP6V1B2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATP6V1B2 in human skeletal muscle using ATP6V1B2 Polyclonal Antibody (Product # PA5-52518) shows low expression as expected.