Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- RZ1130 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Zebrafish Atp6v1b2 Antibody / Atp6v1bb / Vacuolar proton pump subunit B
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Zebrafish
- Host
- Rabbit
- Vial size
- 100 ug
- Concentration
- Lyophilized
- Storage
- After reconstitution, the Zebrafish Atp6v1b2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of Atp6v1b2 protein using Zebrafish Atp6v1b2 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~57 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish brain tissue with Zebrafish Atp6v1b2 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
