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Learnsc-28311
antibody from Santa Cruz Biotechnology
Targeting: GABPA
E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-28311 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-28311, RRID:AB_627648
- Product name
- Anti-GABPA
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Cooperation between NRF-2 and YY-1 transcription factors is essential for triggering the expression of the PREPL-C2ORF34 bidirectional gene pair.
Binding sites for ETS family of transcription factors dominate the promoter regions of differentially expressed genes in abdominal aortic aneurysms.
Huang CC, Chang WS
BMC molecular biology 2009 Jul 3;10:67
BMC molecular biology 2009 Jul 3;10:67
Binding sites for ETS family of transcription factors dominate the promoter regions of differentially expressed genes in abdominal aortic aneurysms.
Nischan J, Gatalica Z, Curtis M, Lenk GM, Tromp G, Kuivaniemi H
Circulation. Cardiovascular genetics 2009 Dec;2(6):565-72
Circulation. Cardiovascular genetics 2009 Dec;2(6):565-72
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- A-431
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 51
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity(granular pattern) in neuronal cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
