PAB29389

antibody from Abnova Corporation

Targeting: GABPA E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29389

Provider

Abnova Corporation

Product name

GABPA polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human GABPA.

Antigen sequence

EELIEIEIDGTEKAECTEESIVEQTYAPAECVSQA
IDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLD
PERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNIL
EIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITL
DGTK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp Lane 3: Human liver tissue Lane 4: Human tonsil tissue with GABPA polyclonal antibody (Cat # PAB29389) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with GABPA polyclonal antibody (Cat # PAB29389) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with GABPA polyclonal antibody (Cat # PAB29389) shows strong nuclear positivity in lymphoid cells outside reaction centra at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)