H00002551-M01

antibody from Abnova Corporation

Targeting: GABPA E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002551-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002551-M01, RRID:AB_425444

Product name

GABPA monoclonal antibody (M01), clone 4B8-G6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GABPA.

Antigen sequence

MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPA
ECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICL
QDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIE
PKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEE
AQVITLDGTKHITTISDETSEQVTRWAAALEGYRK
EQERLGIPYDPIQWSTDQVLHWVVWVMKEFSMTDI
DLTTLNISGRELCSLNQEDFFQRVPRGEILWSHLE
LLRKYVLASQEQQMNEIVTIDQPVQIIPASVQSAT
PTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTG
NNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKL
NQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGD
MICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVTE
CEQKKLAKMQLHGIAQPVTAVALSTASLQTEKDN


Isotype

IgG

Antibody clone number

4B8-G6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GABPA monoclonal antibody (M01), clone 4B8-G6 Western Blot analysis of GABPA expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M01), clone 4B8-G6.Lane 1: GABPA transfected lysate(51.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GABPA is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to GABPA on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol