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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00002551-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002551-M03, RRID:AB_464316
- Product name
- GABPA monoclonal antibody (M03), clone M1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant GABPA.
- Antigen sequence
MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPA
ECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICL
QDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIE
PKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEE
AQVITLDGTKHITTISDETSEQVTRWAAALEGYRK
EQERLGIPYDPIQWSTDQVLHWVVWVMKEFSMTDI
DLTTLNISGRELCSLNQEDFFQRVPRGEILWSHLE
LLRKYVLASQEQQMNEIVTIDQPVQIIPASVQSAT
PTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTG
NNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKL
NQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGD
MICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVTE
CEQKKLAKMQLHGIAQPVTAVALSTASLQTEKDN- Isotype
- IgG
- Antibody clone number
- M1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GABPA monoclonal antibody (M03), clone M1 Western Blot analysis of GABPA expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1.Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GABPA monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
