MA5-15419

antibody from Invitrogen Antibodies

Targeting: GABPA E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A

ELISA

Antibody data

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Validation data

Product number: MA5-15419 - Provider product page
Provider: Invitrogen Antibodies
Product name: GABPA Monoclonal Antibody (8C1B10)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15419 targets GABPA in indirect ELISA, IF ICC and WB applications and shows reactivity with Human and mouse samples. The MA5-15419 immunogen is purified recombinant fragment of human GABPA (aa120-190) expressed in E. Coli. . MA5-15419 detects GABPA which has a predicted molecular weight of approximately 51kDa.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 8C1B10
Vial size: 100 μL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GABPA protein structure - MA5-15419 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GABPA using GABPA monoclonal antibody (Product # MA5-15419) in HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GABPA using GABPA monoclonal antibody (Product # MA5-15419) in HeLa (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-GABPA Monoclonal Antibody (8C1B10) (Product # MA5-15419) and a 55 kDa band corresponding to GABPA was observed across cell lines and tissue extracts tested along with few uncharacterized bands at ~40, ~25 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of NIH/3T3 (Lane 1), HEK-293 (Lane 2) and tissue extracts of Mouse Thymus (Lane 3), Mouse Lung (Lane 4) and Mouse Spleen (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of GABPA was achieved by transfecting NIH/3T3 with GABPA specific siRNAs (Silencer® select Product # s66355, s66356). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the GABPA knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GABPA Monoclonal Antibody (8C1B10) (Product # MA5-15419, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GABPA.