MA5-15419

antibody from Invitrogen Antibodies

Targeting: GABPA E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A

Antibody data

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Validation data

Product number: MA5-15419 - Provider product page
Provider: Invitrogen Antibodies
Product name: GABPA Monoclonal Antibody (8C1B10)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15419 targets GABPA in IF ICC and WB applications and shows reactivity with Human and mouse samples. The MA5-15419 immunogen is purified recombinant fragment of human GABPA (aa120-190) expressed in E. Coli.
Antibody clone number: 8C1B10
Concentration: Conc. Not Determined

GABPA protein structure - MA5-15419 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GABPA using GABPA monoclonal antibody (Product # MA5-15419) in HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GABPA using GABPA monoclonal antibody (Product # MA5-15419) in HeLa (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of GABPA was achieved by transfecting NIH/3T3 with GABPA specific siRNAs (Silencer® select Product # s66355, s66356). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the GABPA knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GABPA Monoclonal Antibody (8C1B10) (Product # MA5-15419, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GABPA.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-GABPA Monoclonal Antibody (8C1B10) (Product # MA5-15419) and a 55 kDa band corresponding to GABPA was observed across cell lines and tissue extracts tested along with few uncharacterized bands at ~40, ~25 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of NIH/3T3 (Lane 1), HEK-293 (Lane 2) and tissue extracts of Mouse Thymus (Lane 3), Mouse Lung (Lane 4) and Mouse Spleen (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).