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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27735 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GABPA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 1.33 mg/mL
Submitted references Contribution of DNA methylation to the expression of FCGRT in human liver and myocardium.
KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis.
Cejas RB, Ferguson DC, Quiñones-Lombraña A, Bard JE, Blanco JG
Scientific reports 2019 Jun 17;9(1):8674
Scientific reports 2019 Jun 17;9(1):8674
KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis.
Qian X, Li X, Shi Z, Bai X, Xia Y, Zheng Y, Xu D, Chen F, You Y, Fang J, Hu Z, Zhou Q, Lu Z
Molecular cell 2019 Dec 19;76(6):885-895.e7
Molecular cell 2019 Dec 19;76(6):885-895.e7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GABPA using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GABPA polyclonal antibody (Product # PA5-27735) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GABPA was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GABPA Polyclonal Antibody (Product # PA5-27735) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GABPA Polyclonal Antibody detects GABPA protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA Polyclonal Antibody (Product # PA5-27735) diluted at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GABPA was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA Polyclonal Antibody (Product # PA5-27735) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GABPA Polyclonal Antibody detects GABPA protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GABPA stained by GABPA Polyclonal Antibody (Product # PA5-27735) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GABPA Polyclonal Antibody detects GABPA protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. GABPA stained by GABPA Polyclonal Antibody (Product # PA5-27735) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
