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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-27735 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GABPA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (97%), Rat (99%), Xenopus laevis (82%), Chicken (86%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.33 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Contribution of DNA methylation to the expression of FCGRT in human liver and myocardium.
KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis.
Cejas RB, Ferguson DC, Quiñones-Lombraña A, Bard JE, Blanco JG
Scientific reports 2019 Jun 17;9(1):8674
Scientific reports 2019 Jun 17;9(1):8674
KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis.
Qian X, Li X, Shi Z, Bai X, Xia Y, Zheng Y, Xu D, Chen F, You Y, Fang J, Hu Z, Zhou Q, Lu Z
Molecular cell 2019 Dec 19;76(6):885-895.e7
Molecular cell 2019 Dec 19;76(6):885-895.e7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GABPA was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA Polyclonal Antibody (Product # PA5-27735) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GABPA was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA Polyclonal Antibody (Product # PA5-27735) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GABPA Polyclonal Antibody detects GABPA protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GABPA stained by GABPA Polyclonal Antibody (Product # PA5-27735) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GABPA Polyclonal Antibody detects GABPA protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. GABPA stained by GABPA Polyclonal Antibody (Product # PA5-27735) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
