PA5-40772

antibody from Invitrogen Antibodies

Targeting: FOXQ1 HFH1

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-40772 - Provider product page
Provider: Invitrogen Antibodies
Product name: FOXQ1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide sequence: MKLEVFVPRA AHGDKQGSDL EGAGGSDAPS PLSAAGDDSL GSDGDCAANS Sequence homology: Human: 100%; Mouse: 85%; Pig: 86%; Rat: 85%
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

FOXQ1 protein structure - PA5-40772 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Foxq1 is highly expressed in NPC tissues, and with a significant correlation with VM formation. A Region showing typical Foxq1 expression in NPC and control; Scale bars represent 50 mum. B Typical morphology of VM (red arrow) and endothelial vessels (black arrow) in NPC visualized using CD31/PAS double staining; Scale bars represent 50 mum. C Statistical results of Foxq1 expression in NPC and control tissues; p < 0.001. D Relationship between Foxq1 grades and VM in NPC tissues; p < 0.001. E Kaplan-Meier survival curves between NPC-positive and negative VM groups; p = 0.046. F - H The relationship between VM and clinical stage ( p = 0.013), T stage ( p = 0.127) and N stage ( p = 0.004) in NPC. Data are presented as mean +- s.d of three independent experiments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5 Foxq1 promotes VM formation, and NPC growth and metastasis through regulating EGFR in vivo. A Image of xenograft tumors from nude mice that were injected with Foxq1 overexpressing or control 5-8F cells (after 2 weeks). B The growth curves of xenograft tumors from each group; p < 0.001. C The weight of xenograft tumors from each group; p < 0.001. D IHC of Foxq1, EGFR and PAS/CD31 double staining of xenograft tumors from each group. Red arrows = VM; Black arrows = endothelial vessels; Scale bars represent 50 mum. E - G Statistical results of Foxq1( p < 0.001),and EGFR ( p < 0.001) expression and VM channels ( p < 0.001) in xenograft tumors from each group. H Metastatic nodules by tail vein injection of each group and normal lung (left). Image of H&E staining of lung sections from each group (right); Scale bars represent 500 mum. I Statistical results of the metastatic nodules in each group; p < 0.001. The expression of related genes in xenograft tumors from each group were monitored by qRT-PCR ( J ) and western blot ( K ). Data are presented as mean +- SD of three independent experiments.