Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-83347 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYH7 Monoclonal Antibody (TH81 (BGN/04/4481))
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Mouse anti Rabbit muscle myosin heavy chain antibody, clone TH81 recognizesthe heavy chain of rabbit muscle myosin.
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TH81 (BGN/04/4481)
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- 4°C or -20°C if preferred
Submitted references RIP3 Contributes to Cardiac Hypertrophy by Influencing MLKL-Mediated Calcium Influx.
Xue H, Shi H, Zhang F, Li H, Li C, Han Q
Oxidative medicine and cellular longevity 2022;2022:5490553
Oxidative medicine and cellular longevity 2022;2022:5490553
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MYH7 Monoclonal Antibody (TH81 (BGN/04/4481)) (Product # MA1-83347) and a 223 kDa band corresponding to MYH7 was observed in Mouse Heart, Rat Heart and Mouse Skeletal Muscle which are reported as positive models when compared to other tissues. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Skeletal Muscle (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Spleen (Lane 6) and Rat Spleen (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
