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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MA5-32986 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYH7 Monoclonal Antibody (NOQ7.5.4D)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- Reconstitute with 1 mL of PBS buffer to yield a concentration of 100 µg/mL.
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- NOQ7.5.4D
- Vial size
- 100 µg
- Concentration
- 100 µg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Cell type-specific genotoxicity in estrogen-exposed ovarian and fallopian epithelium.
Nuclear-mitochondrial communication involving miR-181c plays an important role in cardiac dysfunction during obesity.
ANKK1 is found in myogenic precursors and muscle fibers subtypes with glycolytic metabolism.
Song L, Tang Z, Peng C, Yang Y, Guo C, Wang D, Guo L, Chen J, Liu C
BMC cancer 2020 Oct 21;20(1):1020
BMC cancer 2020 Oct 21;20(1):1020
Nuclear-mitochondrial communication involving miR-181c plays an important role in cardiac dysfunction during obesity.
Roman B, Kaur P, Ashok D, Kohr M, Biswas R, O'Rourke B, Steenbergen C, Das S
Journal of molecular and cellular cardiology 2020 Jul;144:87-96
Journal of molecular and cellular cardiology 2020 Jul;144:87-96
ANKK1 is found in myogenic precursors and muscle fibers subtypes with glycolytic metabolism.
Rubio-Solsona E, Martí S, Vílchez JJ, Palau F, Hoenicka J
PloS one 2018;13(5):e0197254
PloS one 2018;13(5):e0197254
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYH7 in Lane 1: mouse skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with MYH7 monoclonal antibody (Product # MA5-32986) using a 0.5 µg/mL dilution, followed by a goat anti-mouse IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
