AF5777

antibody from Novus Biologicals

Targeting: BACH1 BACH-1, BTBD24

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5777

Provider

Novus Biologicals

Product name

Goat Polyclonal BACH1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous mouse BACH1 in Western blots.

Reactivity

Mouse

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice.

Wang A, Pope SD, Weinstein JS, Yu S, Zhang C, Booth CJ, Medzhitov R
Proceedings of the National Academy of Sciences of the United States of America 2019 Feb 5;116(6):2200-2209

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PGRMC2 is an intracellular haem chaperone critical for adipocyte function.

Galmozzi A, Kok BP, Kim AS, Montenegro-Burke JR, Lee JY, Spreafico R, Mosure S, Albert V, Cintron-Colon R, Godio C, Webb WR, Conti B, Solt LA, Kojetin D, Parker CG, Peluso JJ, Pru JK, Siuzdak G, Cravatt BF, Saez E
Nature 2019 Dec;576(7785):138-142

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse BACH1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5777) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BACH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.