Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 676702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#676702, RRID:AB_2565667
- Product name
- Purified anti-BACH1
- Antibody type
- Monoclonal
- Antigen
- Partial mouse BACH1 recombinant protein (174-415 a.a.) expressed in E. coli.
- Description
- BACH1 is a member of the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip) and forms heterodimers with the bZip subfamily of small Maf proteins such as MafF, MafG, and MafK. The BACH1-Maf heterodimers bind to the Maf recognition element (MARE) in the promoter regions and repress transcription of several genes that are involved in the regulation of oxidative stress. In the absence of oxidative stress, BACH1 was shown to repress transcription of the heme oxygenase-1 (HO-1) gene, which produces the key enzyme of the heme degradation pathway. HO-1 converts heme to ferrous iron, bilirubin, and carbon monoxide, which are potent radical scavengers and possess cytoprotective roles. BACH1 was found to regulate the genes involving in osteolytic metastasis and invasiveness of breast cancer. BACH1 has been suggested to function with BACH2 in promoting B cell development by repressing the myeloid genes in the common lymphoid progenitor cells.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 11D11C25
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from mouse thymus (lane 1), human U251 cells (lane 2), and mouse RAW264.7 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-BACH1 (clone 11D11C25) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated