Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB10027 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10027, RRID:AB_1672167
- Product name
- BACH1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant BACH1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Analysis of the DNA substrate specificity of the human BACH1 helicase associated with breast cancer.
Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Gupta R, Sharma S, Sommers JA, Jin Z, Cantor SB, Brosh RM Jr
The Journal of biological chemistry 2005 Jul 8;280(27):25450-60
The Journal of biological chemistry 2005 Jul 8;280(27):25450-60
Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer.
Clapperton JA, Manke IA, Lowery DM, Ho T, Haire LF, Yaffe MB, Smerdon SJ
Nature structural & molecular biology 2004 Jun;11(6):512-8
Nature structural & molecular biology 2004 Jun;11(6):512-8
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using BACH1 polyclonal antibody (Cat # PAB10027) shows detection of a band at~105 KDa (Lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead).Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction.Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:1,000.Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at roomtemperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).IRDye™800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR,Inc.
