HPA019589

antibody from Atlas Antibodies

Targeting: MIER1 hMI-ER1, KIAA1610, MI-ER1

Immunocytochemistry (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA019589

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA019589, RRID:AB_1853953

Product name

Anti-MIER1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

MMEGETNFSSEIEDLAREGDMPIHELLSLYGYGST
VRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGEN
KEENIKDSSGQEDETQSSNDDPSQSVASQDAQEI


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Enhanced validation

Submitted by

55af80e3e0991

Enhanced method

Genetic validation

Main image

Experimental details

Confocal images of immunofluorescently stained human U-2 OS cells.The protein MIER1 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where MIER1 has been downregulated with specific siRNA.

Sample type

U-2 OS cells

Primary Ab dilution

1:43

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:800

Knockdown/Genetic Approaches Application

Immunocytochemistry

KD Reagent Type

siRNA

Downregulation

<50%

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no

s33645

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no 2

s33643

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Prod no 3

s33644

KD Reagent Page 3

KD Reagent Page 3

Antibody Lot Number

R08028

Appendix

55b89c0581506.pdf

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA019589 antibody. Corresponding MIER1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows weak nuclear positivity in a subset of myocytes.

Sample type

HUMAN