LS-B2996

antibody from LSBio

Targeting: FOXC1 ARA, FKHL7, FREAC3, IGDA, IHG1, IRID1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B2996

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B2996, RRID:AB_2105150

Product name

PathPlus™ FOXC1 Antibody (aa541-553) LS-B2996

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Simian, Xenopus, Zebrafish

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

FOXC1 antibody (0.3µg/ml) staining of HEK293 (A) Nuclear MCF7 (B) and Negative control Daudi (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

FOXC1 antibody (0.5µg/ml) staining of transfected HEK293 transiently expressing full-length Human FOXC1 (myc and DYKDDDDK tagged). Detected by chemiluminescence.