PA5-68774

antibody from Invitrogen Antibodies

Targeting: FOXC1 ARA, FKHL7, FREAC3, IGDA, IHG1, IRID1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-68774

Provider

Invitrogen Antibodies

Product name

FOXC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5-1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-FOXC1 Polyclonal Antibody (Product # PA5-68774) and a 56.7 kDa band corresponding to FOXC1 was observed across all cell lines tested, except MCF10A and MIA PaCa-2. whole cell extracts (40 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with IL-8 (50 ng/mL, 24hrs) (Lane 2), A549 (Lane 3), MCF10A (Lane 4), MIA PaCa-2 (Lane 5) were electrophoresed using NuPAGE™10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34075).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FOXC1 on mouse NIH-3T3 cells. The sample was probed with a FOXC1 polyclonal antibody (Product # PA5-68774) using a primary antibody dilution of 0.2-1.0 µg/mL.