A303-519A

antibody from Invitrogen Antibodies

Targeting: FOXC1 ARA, FKHL7, FREAC3, IGDA, IHG1, IRID1

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-519A

Provider

Invitrogen Antibodies

Product name

FOXC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FOXC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- FOXC1 (Cat. No. A303-519A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FOXC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-FOXC1 antibody A303-519A used for IP at 6 µg/mg lysate. FOXC1 was also immunoprecipitated by rabbit anti-FOXC1 antibody A303-520A, which recognizes a downstream epitope. For blotting immunoprecipitated FOXC1, A303-520A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.