LS-C348730

antibody from LSBio

Targeting: NEUROD1 BETA2, BHF-1, bHLHa3, MODY6, NEUROD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C348730

Provider

LSBio

Product name

Anti-NEUROD1 Antibody (clone BC18-1.11EG) LS-C348730

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

BC18-1.11EG

Vial size

100µg

Storage

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Figure 3. Shows reactivity of anti-NEUROD1 Antibody (Clone BC18-1.11EG) against SHSY5Y, Kelly and SKNSH neuroblastoma cell lysates by Western blotting. For each cell line, a band of approximately 45kD was observed. This molecular size corresponds to the expected size for NEUROD1. The antibody was used at 2 ug/ml and incubated overnight at 4°C The peroxidase conjugated anti-Mouse Ig secondary antibody was used at 1/10000 dilution and incubated for 2 hours. The blot was developed using enhanced chemiluminescence and image captured on radiographic films.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Figure 2. Shows K562 (chronic myelogenous leukemia), RDES (Ewings sarcoma), SKES (Ewings) sarcoma and PFSK1 (Primitive neuroectodermal tumor) cells stained with anti-NEUROD1 Antibody (Clone BC18-1.11EG) by the indirect immunoperoxidase method. K562 was used as a negative control and showed no staining whereas RDES, SKES-1 and PFSK-1 cells showed weak nuclear but strong cytoplasmic staining. Since the cell lines tested loosely attach to surfaces of chamber slides, the cells were immobilized on adhesion slides for the staining procedure. The cells were fixed in 1% paraformaldehyde, permeabilized in 0.25% Triton X 100 in PBS, blocked with 1% BSA and stained overnight with 5 ug/ml antibody diluted with 1% BSA in PBS. The peroxidase conjugated anti Mouse Ig secondary antibody was used at 1:1000 in 1% BSA in PBS. DAB substrate was used with nickel chloride enhancement to give dark purple/black staining for positive reaction. The substrate was added for 10 minutes, extensively washed, counterstained in diluted hematoxylin, dehydrated, cleared in xylene and mounted.