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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- F47842 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NeuroD1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific NeuroD1 antibody is suitable for use in Western blot/Immunofluorescence/Immunohistochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the NeuroD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of NeuroD1 and HepG2 lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HepG2 cells stained with NeuroD1 antibody. Alexa Fluor 488 conjugated secondary (green) was used. Cytoplasmic actin (red) and nuclei (blue) were counterstained. NeuroD1 immunoreactivity is localized to vesicles.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ES cells were transiently transfected with Flag-tagged mouse NeuroD1 (tagged on N-term), fixed 24h post transfection and stained for flag tag (red) to check expression. NeuroD1 antibody (1:100) showed predominantly nuclear staining wtih some cytoplasmic.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with the NeuroD1 antibody
