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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF2746 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse NeuroD1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13562- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development.
Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3.
Sharon N, Vanderhooft J, Straubhaar J, Mueller J, Chawla R, Zhou Q, Engquist EN, Trapnell C, Gifford DK, Melton DA
Cell reports 2019 May 21;27(8):2281-2291.e5
Cell reports 2019 May 21;27(8):2281-2291.e5
Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3.
Farioli-Vecchioli S, Tanori M, Micheli L, Mancuso M, Leonardi L, Saran A, Ciotti MT, Ferretti E, Gulino A, Pazzaglia S, Tirone F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Jul;21(9):2215-25
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Jul;21(9):2215-25
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- NeuroD1 in beta TC-6 Mouse Cell Line. Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
