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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX107574 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107574, RRID:AB_2037881
- Product name
- RIP3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
Submitted references Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients.
Corilagin inhibits breast cancer growth via reactive oxygen species-dependent apoptosis and autophagy.
SP110b Controls Host Immunity and Susceptibility to Tuberculosis.
DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.
Galangin ameliorates cisplatin-induced nephrotoxicity by attenuating oxidative stress, inflammation and cell death in mice through inhibition of ERK and NF-kappaB signaling.
Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells.
Immune-Mediated Nephropathy and Systemic Autoimmunity in Mice Does Not Require Receptor Interacting Protein Kinase 3 (RIPK3).
Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis.
Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis.
Resveratrol alleviates the cytotoxicity induced by the radiocontrast agent, ioxitalamate, by reducing the production of reactive oxygen species in HK-2 human renal proximal tubule epithelial cells in vitro.
Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Activation-induced necroptosis contributes to B-cell lymphopenia in active systemic lupus erythematosus.
Corsetti G, Chen-Scarabelli C, Romano C, Pasini E, Dioguardi FS, Onorati F, Knight R, Patel H, Saravolatz L, Faggian G, Scarabelli TM
Medical science monitor basic research 2019 Feb 4;25:33-44
Medical science monitor basic research 2019 Feb 4;25:33-44
Corilagin inhibits breast cancer growth via reactive oxygen species-dependent apoptosis and autophagy.
Tong Y, Zhang G, Li Y, Xu J, Yuan J, Zhang B, Hu T, Song G
Journal of cellular and molecular medicine 2018 Aug;22(8):3795-3807
Journal of cellular and molecular medicine 2018 Aug;22(8):3795-3807
SP110b Controls Host Immunity and Susceptibility to Tuberculosis.
Leu JS, Chen ML, Chang SY, Yu SL, Lin CW, Wang H, Chen WC, Chang CH, Wang JY, Lee LN, Yu CJ, Kramnik I, Yan BS
American journal of respiratory and critical care medicine 2017 Feb 1;195(3):369-382
American journal of respiratory and critical care medicine 2017 Feb 1;195(3):369-382
DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.
Tsui KH, Wang PH, Lin LT, Li CJ
Reproduction (Cambridge, England) 2017 Aug;154(2):101-110
Reproduction (Cambridge, England) 2017 Aug;154(2):101-110
Galangin ameliorates cisplatin-induced nephrotoxicity by attenuating oxidative stress, inflammation and cell death in mice through inhibition of ERK and NF-kappaB signaling.
Huang YC, Tsai MS, Hsieh PC, Shih JH, Wang TS, Wang YC, Lin TH, Wang SH
Toxicology and applied pharmacology 2017 Aug 15;329:128-139
Toxicology and applied pharmacology 2017 Aug 15;329:128-139
Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells.
Lin CY, Chang TW, Hsieh WH, Hung MC, Lin IH, Lai SC, Tzeng YJ
Cell death discovery 2016;2:16065
Cell death discovery 2016;2:16065
Immune-Mediated Nephropathy and Systemic Autoimmunity in Mice Does Not Require Receptor Interacting Protein Kinase 3 (RIPK3).
Corradetti C, Jog NR, Gallucci S, Madaio M, Balachandran S, Caricchio R
PloS one 2016;11(9):e0163611
PloS one 2016;11(9):e0163611
Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis.
Wang G, Liu X, Gaertig MA, Li S, Li XJ
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar 22;113(12):3359-64
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar 22;113(12):3359-64
Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis.
Al-Lamki RS, Lu W, Manalo P, Wang J, Warren AY, Tolkovsky AM, Pober JS, Bradley JR
Cell death & disease 2016 Jun 30;7(6):e2287
Cell death & disease 2016 Jun 30;7(6):e2287
Resveratrol alleviates the cytotoxicity induced by the radiocontrast agent, ioxitalamate, by reducing the production of reactive oxygen species in HK-2 human renal proximal tubule epithelial cells in vitro.
Huang YT, Chen YY, Lai YH, Cheng CC, Lin TC, Su YS, Liu CH, Lai PC
International journal of molecular medicine 2016 Jan;37(1):83-91
International journal of molecular medicine 2016 Jan;37(1):83-91
Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Li CJ, Sun LY, Pang CY
Scientific reports 2015 Apr 24;5:9819
Scientific reports 2015 Apr 24;5:9819
Activation-induced necroptosis contributes to B-cell lymphopenia in active systemic lupus erythematosus.
Fan H, Liu F, Dong G, Ren D, Xu Y, Dou J, Wang T, Sun L, Hou Y
Cell death & disease 2014 Sep 11;5:e1416
Cell death & disease 2014 Sep 11;5:e1416
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30?g whole cell lysate)A:A431(GTX27909)B:H12997.5% SDS PAGEGTX107574 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (60 ?g of whole cell lysate) A: mouse B lymphocyte B: mouse thymocyte C: mouse T lymphocyte GTX107574 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with RIP3 antibody (GTX107574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RIP3 antibody detects RIP3 protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP3 antibody (GTX107574) diluted by 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RIP3 (GTX107574) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RIP3 antibody detects RIP3 protein at cytoplasm in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon. RIP3 antibody (GTX107574) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RIP3 antibody detects RIP3 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RIP3 antibody (GTX107574) diluted at 1:500.
