PA5-19956

antibody from Invitrogen Antibodies

Targeting: RIPK3 RIP3

Western blot (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19956

Provider

Invitrogen Antibodies

Product name

RIP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is NIH-3T3 cell lysate. PA5-19956 can be used with blocking peptide PEP-0081. The PA5-19956 immunogen is located within the last 50 amino acids of RIP3. Predicted molecular ~ 53kD for mouse RIP3 and 57kD for human RIP3. In Western blot applications, this antibody has been observed to detect a band at: 53kD for mouse RIP3 and 57kD for human RIP3

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C

References

Submitted references

Therapy With Carboplatin and Anti-PD-1 Antibodies Before Surgery Demonstrates Sustainable Anti-Tumor Effects for Secondary Cancers in Mice With Triple-Negative Breast Cancer.

Gao M, Wang T, Ji L, Bai S, Tian L, Song H
Frontiers in immunology 2020;11:366

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Autophosphorylation at serine 166 regulates RIP kinase 1-mediated cell death and inflammation.

Laurien L, Nagata M, Schünke H, Delanghe T, Wiederstein JL, Kumari S, Schwarzer R, Corona T, Krüger M, Bertrand MJM, Kondylis V, Pasparakis M
Nature communications 2020 Apr 8;11(1):1747

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed Rat Kidney tissue labeling RIP3 with RIP3 Polyclonal Antibody (Product # PA5-19956) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using RIP3 Polyclonal Antibody (Product # PA5-19956) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using RIP3 Polyclonal Antibody (Product # PA5-19956) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 S166 phosphorylation enhances RIPK1 kinase activity. a Scheme depicting the possible combinations of RIPK1 dimers in Ripk1 D138N/S166A mice. Residue 166 on RIPK1 is indicated. b , BMDMs from mice of the indicated genotypes were treated with a combination of TNF (T; 10 ng mL -1 ), SMAC mimetic (S (Birinapant), 1 muM) and Z-VAD-FMK (Z) for 0, 2 or 4 h and lysates were analyzed by immunoblot with the indicated antibodies. Blots shown are representative of three independent experiments. *Non-specific band. c Primary lung fibroblasts from mice of the indicated genotypes were treated with a combination of TNF (T; 20 ng mL -1 ), SMAC mimetic (S (Birinapant), 1 muM) and Z-VAD-FMK (Z) for 0, 3 or 5 h and lysates were analyzed by immunoblot with the indicated antibodies. Blots shown are representative of three independent experiments. d BMDMs from mice of the indicated genotypes were treated with combinations of TNF (T; 10 ng mL -1 ), SMAC mimetic (S (Briniapant), 1 muM) and Z-VAD-FMK (Z) in the presence or absence of Nec-1s. Cell death was measured using IncuCyte. Graph shows mean of technical triplicates. Data are representative of three independent experments. For purpose of comparability the data of the genotypes WT, Ripk1 S166A/S166A and Ripk1 D138N/D138N shown here are identical to those in Fig. 1b . e BMDMs were treated with a combination of TNF (T; 10 ng mL -1 ), SMAC mimetic (S (Birinapant), 1 muM) and Z-VAD-FMK (Z) for 0, 2 or 4 h. FADD immunoprecipitates and total cel