F41536

antibody from NSJ Bioreagents

Targeting: MITF bHLHe32, MI, WS2, WS2A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F41536

Provider

NSJ Bioreagents

Product name

MITF Antibody

Antibody type

Polyclonal

Description

This highly specific MITF antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the MITF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of MITF antibody at 1:2000 dilution + A375 lysate; Predicted molecular weight: 55-60 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

MITF antibody western blot analysis in K562 lysate. Predicted molecular weight: 55-60 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of MITF antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

MITF antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram)