HPA003259

MITF antibody from Atlas Antibodies

bHLHe32, MI, WS2, WS2A

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for HPA003259

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Antibody Data

Product number

HPA003259

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003259, RRID:AB_1079381

Product name

Anti-MITF

Provider product page

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

HLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRI
IKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTIT
FNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLS
PVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHT


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

MITF and c-Jun antagonism interconnects melanoma dedifferentiation with pro-inflammatory cytokine responsiveness and myeloid cell recruitment
Riesenberg S, Groetchen A, Siddaway R, Bald T, Reinhardt J, Smorra D, Kohlmeyer J, Renn M, Phung B, Aymans P, Schmidt T, Hornung V, Davidson I, Goding C, Jönsson G, Landsberg J, Tüting T, Hölzel M
Nature Communications 2015 November;6

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Enhancer-targeted genome editing selectively blocks innate resistance to oncokinase inhibition
Webster D, Barajas B, Bussat R, Yan K, Neela P, Flockhart R, Kovalski J, Zehnder A, Khavari P
Genome Research 2014 May;24(5):751-760

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-MITF antibody. Corresponding MITF RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and MITF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404987).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows moderate to strong nuclear positivity in melanocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human melanoma shows strong nuclear positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows moderate nuclear positivity in cells in endometrial stroma.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows no positivity as expected.

Sample type

HUMAN