HPA003259

antibody from Atlas Antibodies

Targeting: MITF bHLHe32, MI, WS2, WS2A

Provider product page for HPA003259

Western blot

Immunohistochemistry

Chromatin Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Immunohistochemistry [1]
Chromatin Immunoprecipitation [1]

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Product number: HPA003259 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA003259, RRID:AB_1079381
Product name: Anti-MITF
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human MITF, Gene description: microphthalmia-associated transcription factor, Alternative Gene Names: bHLHe32, MI, WS2, WS2A, Validated applications: ChIP, IHC, WB, Uniprot ID: O75030, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.1 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

MITF protein structure - HPA003259 shown in red.

Submitted references DNA damage remodels the MITF interactome to increase melanoma genomic instability

Binet R, Lambert J, Tomkova M, Tischfield S, Baggiolini A, Picaud S, Sarkar S, Louphrasitthiphol P, Dias D, Carreira S, Humphrey T, Fillipakopoulos P, White R, Goding C
Genes & Development 2024;38(1-2):70-94

Comparative Analysis of Olive-Derived Phenolic Compounds’ Pro-Melanogenesis Effects on B16F10 Cells and Epidermal Human Melanocytes

Cho J, Bejaoui M, Tominaga K, Isoda H
International Journal of Molecular Sciences 2024;25(8):4479

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma

Thornock A, Noonan H, Barbano J, Xifaras M, Baron C, Yang S, Koczirka K, McConnell A, Zon L
eLife 2024;13

Chang J, Campbell-Hanson K, Vanneste M, Yevdash J, Bartschat N, Jiarui J, Bhinu A, Helverson A, Henry M, Steingrimsson E, Weigel R, Cornell R, Kenny C
2024

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions

Skinner O, Blanco-Fernández J, Goodman R, Kawakami A, Shen H, Kemény L, Joesch-Cohen L, Rees M, Roth J, Fisher D, Mootha V, Jourdain A
Nature Metabolism 2023;5(5):765-776

Lycopene inhibits endothelial‐to‐mesenchymal transition of choroidal vascular endothelial cells in laser‐induced mouse choroidal neovascularization

Li L, Cao X, Huang L, Huang X, Gu J, Yu X, Zhu Y, Zhou Y, Song Y, Zhu M
Journal of Cellular and Molecular Medicine 2023;27(10):1327-1340

Binet R, Lambert J, Tomkova M, Tischfield S, Baggiolini A, Picaud S, Sarkar S, Louphrasitthiphol P, Dias D, Carreira S, Humphrey T, Fillipakopoulos P, White R, Goding C
2023

A cellular hierarchy in melanoma uncouples growth and metastasis

Karras P, Bordeu I, Pozniak J, Nowosad A, Pazzi C, Van Raemdonck N, Landeloos E, Van Herck Y, Pedri D, Bervoets G, Makhzami S, Khoo J, Pavie B, Lamote J, Marin-Bejar O, Dewaele M, Liang H, Zhang X, Hua Y, Wouters J, Browaeys R, Bergers G, Saeys Y, Bosisio F, van den Oord J, Lambrechts D, Rustgi A, Bechter O, Blanpain C, Simons B, Rambow F, Marine J
Nature 2022;610(7930):190-198

MITF activity is regulated by a direct interaction with RAF proteins in melanoma cells

Estrada C, Mirabal-Ortega L, Méry L, Dingli F, Besse L, Messaoudi C, Loew D, Pouponnot C, Bertolotto C, Eychène A, Druillennec S
Communications Biology 2022;5(1)

The Critical Role Played by Mitochondrial MITF Serine 73 Phosphorylation in Immunologically Activated Mast Cells

Paruchuru L, Govindaraj S, Razin E
Cells 2022;11(3):589

Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress.

Fessé P, Nyman J, Hermansson I, Book ML, Ahlgren J, Turesson I
iScience 2022 Oct 21;25(10):105238

Activation of the integrated stress response confers vulnerability to mitoribosome-targeting antibiotics in melanoma

Vendramin R, Katopodi V, Cinque S, Konnova A, Knezevic Z, Adnane S, Verheyden Y, Karras P, Demesmaeker E, Bosisio F, Kucera L, Rozman J, Gladwyn-Ng I, Rizzotto L, Dassi E, Millevoi S, Bechter O, Marine J, Leucci E
Journal of Experimental Medicine 2021;218(9)

TOP1 modulation during melanoma progression and in adaptative resistance to BRAF and MEK inhibitors

Oliveira É, Chauhan J, Silva J, Carvalho L, Dias D, Carvalho D, Watanabe L, Rebecca V, Mills G, Lu Y, da Silva A, Consolaro M, Herlyn M, Possik P, Goding C, Maria-Engler S
Pharmacological Research 2021;173

BRN2 is a non-canonical melanoma tumor-suppressor

Hamm M, Sohier P, Petit V, Raymond J, Delmas V, Le Coz M, Gesbert F, Kenny C, Aktary Z, Pouteaux M, Rambow F, Sarasin A, Charoenchon N, Bellacosa A, Sanchez-del-Campo L, Mosteo L, Lauss M, Meijer D, Steingrimsson E, Jönsson G, Cornell R, Davidson I, Goding C, Larue L
Nature Communications 2021;12(1)

MITF reprograms the extracellular matrix and focal adhesion in melanoma

Dilshat R, Fock V, Kenny C, Gerritsen I, Lasseur R, Travnickova J, Eichhoff O, Cerny P, Möller K, Sigurbjörnsdóttir S, Kirty K, Einarsdottir B, Cheng P, Levesque M, Cornell R, Patton E, Larue L, de Tayrac M, Magnúsdóttir E, Ögmundsdóttir M, Steingrimsson E
eLife 2021;10

Classification and Grading of Melanocytic Lesions in a Mouse Model of NRAS-driven Melanomagenesis.

Assenmacher CA, Santagostino SF, Oyama MA, Marine JC, Bonvin E, Radaelli E
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2021 Mar;69(3):203-218

MITF is a driver oncogene and potential therapeutic target in kidney angiomyolipoma tumors through transcriptional regulation of CYR61

Zarei M, Giannikou K, Du H, Liu H, Duarte M, Johnson S, Nassar A, Widlund H, Henske E, Long H, Kwiatkowski D
Oncogene 2020;40(1):112-126

PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.

Johansson JA, Marie KL, Lu Y, Brombin A, Santoriello C, Zeng Z, Zich J, Gautier P, von Kriegsheim A, Brunsdon H, Wheeler AP, Dreger M, Houston DR, Dooley CM, Sims AH, Busch-Nentwich EM, Zon LI, Illingworth RS, Patton EE
Developmental cell 2020 Aug 10;54(3):317-332.e9

Molecular phenotyping and image-guided surgical treatment of melanoma using spectrally distinct ultrasmall core-shell silica nanoparticles

Chen F, Madajewski B, Ma K, Karassawa Zanoni D, Stambuk H, Turker M, Monette S, Zhang L, Yoo B, Chen P, Meester R, Jonge S, Montero P, Phillips E, Quinn T, Gönen M, Sequeira S, de Stanchina E, Zanzonico P, Wiesner U, Patel S, Bradbury M
Science Advances 2019;5(12)

MITF controls the TCA cycle to modulate the melanoma hypoxia response

Louphrasitthiphol P, Ledaki I, Chauhan J, Falletta P, Siddaway R, Buffa F, Mole D, Soga T, Goding C
Pigment Cell & Melanoma Research 2019;32(6):792-808

The MITF-SOX10 regulated long non-coding RNA DIRC3 is a melanoma tumour suppressor

Widlund H, Coe E, Tan J, Shapiro M, Louphrasitthiphol P, Bassett A, Marques A, Goding C, Vance K
PLOS Genetics 2019;15(12):e1008501

A direct link between MITF, innate immunity, and hair graying

Yamashita Y, Harris M, Fufa T, Palmer J, Joshi S, Larson D, Incao A, Gildea D, Trivedi N, Lee A, Day C, Michael H, Hornyak T, Merlino G, Pavan W
PLOS Biology 2018;16(5):e2003648

Lysosomal adaptation: How cells respond to lysosomotropic compounds

Komatsu M, Lu S, Sung T, Lin N, Abraham R, Jessen B
PLOS ONE 2017;12(3):e0173771

BMP-induced reprograming of the retina into RPE requires WNT signalling in the developing chick optic cup

Steinfeld J, Steinfeld I, Bausch A, Coronato N, Hampel M, Depner H, Layer P, Vogel-Höpker A
Biology Open 2017

MITF and c-Jun antagonism interconnects melanoma dedifferentiation with pro-inflammatory cytokine responsiveness and myeloid cell recruitment.

Riesenberg S, Groetchen A, Siddaway R, Bald T, Reinhardt J, Smorra D, Kohlmeyer J, Renn M, Phung B, Aymans P, Schmidt T, Hornung V, Davidson I, Goding CR, Jönsson G, Landsberg J, Tüting T, Hölzel M
Nature communications 2015 Nov 4;6:8755

Enhancer-targeted genome editing selectively blocks innate resistance to oncokinase inhibition

Webster D, Barajas B, Bussat R, Yan K, Neela P, Flockhart R, Kovalski J, Zehnder A, Khavari P
Genome Research 2014;24(5):751-760

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human skin and pancreas tissues using HPA003259 antibody. Corresponding MITF RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: ChIP-Exo-Seq composite graph for Anti-MITF (HPA003259, Lot 000014341) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.