AP7365A

antibody from Abgent

Targeting: SMAD2 JV18-1, MADH2, MADR2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7365A

Provider

Abgent

Proper citation

Abgent Cat#AP7365a, RRID:AB_1968304

Product name

SMAD2 Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of SMAD2 Antibody (Cat.# AP7365a) in NIH-3T3 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP7365a SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus."

Primary Ab dilution

1:100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

SMAD2 Antibody (Cat. #AP7365a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50