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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NBP2-37580 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Smad2 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against human SMAD2 (AA: 20-254) recombinant protein. (Expected MW is 52.2 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Smad2 Antibody (5G7) [NBP2-37580] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using SMAD2 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using SMAD2 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Smad2 Antibody (5G7) [NBP2-37580] - Flow cytometric analysis of NIH/3T3 cells using SMAD2 mouse mAb (blue) and negative control (red).
