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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ARG54119 - Provider product page
- Provider
- Arigo
- Product name
- anti-Smad2 antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human Smad2 protein fragments expressed in E.coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6H5-E3-C11
- Vial size
- 100 µl
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Smad2 in HeLa, A431, Jurkat and K562 cell lysates stained with Smad2 antibody [6H5-E3-C11] (ARG54119) (diluted in 1: 500). Predicted band size: 60KDa. Observed band size: 60KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with 1% Paraformaldehyde and stained with Smad2 antibody [6H5-E3-C11] (ARG54119) (diluted in 1:100).
