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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- F48613 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phospho-Smad2 Antibody
- Antibody type
- Polyclonal
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells/lysate, untreated or treated with PMA, using phospho-SMAD2 antibody (left) or nonphos Ab (right).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of phospho-SMAD2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Dot blot analysis of phospho-SMAD2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
