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Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Proximity ligation assay [1]
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- Product number
- H00004087-M17 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004087-M17, RRID:AB_1204990
- Product name
- SMAD2 monoclonal antibody (M17), clone 4D4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant SMAD2.
- Antigen sequence
LGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVK
KLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIW
GLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSH- Isotype
- IgG
- Antibody clone number
- 4D4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between TP53 and SMAD2. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-SMAD2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
