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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- M00090-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Smad2 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for SMAD2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, ICC, WB. Reactive species: Human, Mouse, Rat SMAD2 information: Molecular Weight: 52306 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1; Tissue Specificity: Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- ACI-19
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1.
Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells.
The effects of endosulfan on cell migration and invasion in prostate cancer cells via the KCNQ1OT1/miR-137-3p/PTP4A3 axis.
Enhanced transforming growth factor-beta signaling and fibrosis in the pectoralis major muscle of broiler chickens affected by wooden breast myopathy.
Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway.
Zhao J, Liu X, Jin X, Dong T, Gao X, Wang J, Li Y, Ma E
Bioorganic chemistry 2024 May;146:107274
Bioorganic chemistry 2024 May;146:107274
Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells.
Zhang B, Liu S, Sun Y, Xu D
Toxicology 2023 Jan 15;484:153392
Toxicology 2023 Jan 15;484:153392
The effects of endosulfan on cell migration and invasion in prostate cancer cells via the KCNQ1OT1/miR-137-3p/PTP4A3 axis.
Wang Y, Guo Y, Lu Y, Sun Y, Xu D
The Science of the total environment 2022 Nov 1;845:157252
The Science of the total environment 2022 Nov 1;845:157252
Enhanced transforming growth factor-beta signaling and fibrosis in the pectoralis major muscle of broiler chickens affected by wooden breast myopathy.
Xing T, Zhao ZR, Zhao X, Xu XL, Zhang L, Gao F
Poultry science 2021 Mar;100(3):100804
Poultry science 2021 Mar;100(3):100804
Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway.
Wang W, Zha G, Zou JJ, Wang X, Li CN, Wu XJ
Current medical science 2019 Oct;39(5):748-753
Current medical science 2019 Oct;39(5):748-753
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rat stomach, using Smad2 Antibody(M00090-1)SMAD2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (M00090-1)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
