A00090-1

antibody from Boster Biological Technology

Targeting: SMAD2 JV18-1, MADH2, MADR2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A00090-1

Provider

Boster Biological Technology

Product name

Anti-SMAD2 Antibody

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for SMAD2 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

MiR-132-3p suppresses peritoneal fibrosis induced by peritoneal dialysis via targeting TGF-β1/Smad2/3 signaling pathway.

Yin Y, Yang Y, Zhang Y, Shang Y, Li Q, Yuan J
PloS one 2024;19(4):e0301540

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miR-140-5p attenuates hepatic fibrosis by directly targeting TGFβR1.

Pan W, Wang Y, Zhao C
Scandinavian journal of gastroenterology 2023 Jul-Dec;58(11):1335-1343

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Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells.

Zhang B, Liu S, Sun Y, Xu D
Toxicology 2023 Jan 15;484:153392

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The effects of endosulfan on cell migration and invasion in prostate cancer cells via the KCNQ1OT1/miR-137-3p/PTP4A3 axis.

Wang Y, Guo Y, Lu Y, Sun Y, Xu D
The Science of the total environment 2022 Nov 1;845:157252

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Enhanced transforming growth factor-beta signaling and fibrosis in the pectoralis major muscle of broiler chickens affected by wooden breast myopathy.

Xing T, Zhao ZR, Zhao X, Xu XL, Zhang L, Gao F
Poultry science 2021 Mar;100(3):100804

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Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis.

Xu X, Xu C, Saud SM, Lu X, Liu L, Fang L, Zhang X, Hu J, Li W
Evidence-based complementary and alternative medicine : eCAM 2016;2016:2601830

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysatesLane 2: rat liver tissue lysatesLane 3: mouse testicular tissue lysatesLane 4: mouse heart tissue lysatesLane 5: mouse lung tissue lysatesLane 6: mouse lung tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60KD. The expected band size for SMAD2 is at 52KD.

Additional image