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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
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- Product number
- PA1898 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Smad2 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for SMAD2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. SMAD2 information: Molecular Weight: 52306 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1; Tissue Specificity: Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1.
Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells.
The effects of endosulfan on cell migration and invasion in prostate cancer cells via the KCNQ1OT1/miR-137-3p/PTP4A3 axis.
Hirudin Ameliorates Renal Interstitial Fibrosis via Regulating TGF-β1/Smad and NF-κB Signaling in UUO Rat Model.
Endosulfan triggers epithelial-mesenchymal transition via PTP4A3-mediated TGF-β signaling pathway in prostate cancer cells.
Gremlin1 promotes carcinogenesis of glioma in vitro.
Zhao J, Liu X, Jin X, Dong T, Gao X, Wang J, Li Y, Ma E
Bioorganic chemistry 2024 May;146:107274
Bioorganic chemistry 2024 May;146:107274
Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells.
Zhang B, Liu S, Sun Y, Xu D
Toxicology 2023 Jan 15;484:153392
Toxicology 2023 Jan 15;484:153392
The effects of endosulfan on cell migration and invasion in prostate cancer cells via the KCNQ1OT1/miR-137-3p/PTP4A3 axis.
Wang Y, Guo Y, Lu Y, Sun Y, Xu D
The Science of the total environment 2022 Nov 1;845:157252
The Science of the total environment 2022 Nov 1;845:157252
Hirudin Ameliorates Renal Interstitial Fibrosis via Regulating TGF-β1/Smad and NF-κB Signaling in UUO Rat Model.
Yang K, Fan B, Zhao Q, Ji Y, Liu P, Gao S, Ren T, Dou Y, Pei M, Yang H
Evidence-based complementary and alternative medicine : eCAM 2020;2020:7291075
Evidence-based complementary and alternative medicine : eCAM 2020;2020:7291075
Endosulfan triggers epithelial-mesenchymal transition via PTP4A3-mediated TGF-β signaling pathway in prostate cancer cells.
Wang Y, Guo Y, Hu Y, Sun Y, Xu D
The Science of the total environment 2020 Aug 20;731:139234
The Science of the total environment 2020 Aug 20;731:139234
Gremlin1 promotes carcinogenesis of glioma in vitro.
Guan Y, Cheng W, Zou C, Wang T, Cao Z
Clinical and experimental pharmacology & physiology 2017 Feb;44(2):244-256
Clinical and experimental pharmacology & physiology 2017 Feb;44(2):244-256
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti-Smad2 antibody, PA1898, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD (162aa tag+D424-P578)Lane 1: Recombinant Human SHC1 Proteins 10ng Lane 2: Recombinant Human SHC1 Proteins 5ng Lane 3: Recombinant Human SHC1 Proteins 2.5ng
- Additional image
