LS-C54764

antibody from LSBio

Targeting: ELF3 EPR-1, ERT, ESE-1, ESX

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C54764

Provider

LSBio

Product name

ELF3 / ESE1 Antibody (aa336-348) LS-C54764

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Simian

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody staining (3 ug/ml) of NCI-H460 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody staining (3 ug/ml) of NCI-H460 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293 overexpressing ELF3 (RC200631) and probed with (mock transfection in first lane).