MAB5787

antibody from Novus Biologicals

Targeting: ELF3 EPR-1, ERT, ESE-1, ESX

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5787

Provider

Novus Biologicals

Product name

Mouse Monoclonal ELF3/ESE-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse ELF3 in Western blots.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse ELF3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, EL-4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ELF3 Monoclonal Antibody (Catalog # MAB5787) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.