Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47776 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ELF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of human/mouse ELF3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ELF3 in PC‚3 human prostate cancer cell line, A549 human lung carcinoma cell line, and NIH‚3T3 mouse embryonic fibroblast cell line . Samples were incubated in ELF3 polyclonal antibody (Product # PA5-47776) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ELF3 in immersion fixed A431 human epithelial carcinoma cell line. Samples were incubated in ELF3 polyclonal antibody (Product # PA5-47776) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ELF3 in immersion fixed paraffin-embedded sections of human liver. Samples were incubated in ELF3 polyclonal antibody (Product # PA5-47776) using a dilution of 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).