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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 630201 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#630201, RRID:AB_2096636
- Product name
- Purified anti-eIF3/p116
- Antibody type
- Polyclonal
- Antigen
- Recombinant human eIF3/p116 (full length)
- Description
- The eIF3/p116 polyclonal antibody recognizes human eIF3/p116 also known as eukaryotic translation initiation factor 3, subunit 9 eta 116 kD, EIF3 ETA, EIF3 P110, eIF3b, and PRT1. eIF3/p116 is a member of the eukaryotic translation initiation factor 3 family. eIF-3 is the largest of the eIFs and is composed of at least 12 different subunits; eIF3/p116 is one such subunit with an apparent molecular weight of 116 kD. eIF3/p116 is a ubiquitously expressed ribosome protein that binds to the 40s ribosome and promotes the binding of methionyl-tRNA and mRNA. eIF3/p116 has been shown to interact with other subunits of EIF-3, IL-2Rβ, and TRIP1 protein. The eIF3/p116 antibody has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 50 µl
- Storage
- Upon receipt, store frozen at -20° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with polyclonal ant-eIF/p116 antibody. Proteins were visualized using a goat anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
