NBP1-87945

antibody from Novus Biologicals

Targeting: ELF3 EPR-1, ERT, ESE-1, ESX

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-87945

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-87945, RRID:AB_11036348

Product name

Rabbit Polyclonal ELF3/ESE-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human ELF3/ESE-1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-ELF3 antibody. Corresponding ELF3 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in control (vector only transfected HEK293T lysate) and ELF3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human fallopian tube shows nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human pancreas shows moderate nuclear positivity in pancreatic ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining in human small intestine and skeletal muscle tissues . Corresponding ELF3 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human liver.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human colon and skeletal muscle tissues. Corresponding ELF3 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon, endometrium, pancreas and skeletal muscle using Anti-ELF3 antibody NBP1-87945 (A) shows similar protein distribution across tissues to independent antibody NBP1-87946 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon, endometrium, pancreas and skeletal muscle using Anti-ELF3/ESE-1 antibody NBP1-87945 (A) shows similar protein distribution across tissues to independent antibody NBP1-87946 (B).