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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-46984 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Brachyury Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Brachyury in 10 ng recombinant Human Brachyury (amino acids Ser2-Glu202) and recombinant Human TBX6. Samples were incubated in Brachyury polyclonal antibody (Product # PA5-46984) using a dilution of 0.1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Brachyury in lysates of NCI-H460 human large cell lung carcinoma cell line and MCF 10A human breast epithelial cell line (negative control). Samples were incubated in Brachyury polyclonal antibody (Product # PA5-46984) using a dilution of 1 µg/mL followed by HRP-conjugated Anti-Goat IgG. A specific band was detected for Brachyury at approximately 50 kDa (as indicated). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-human Brachyury Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46984) at 10 µg/mL for 3 hours at room temperature. Cells were stained using a 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Brachyury in immersion fixed differentiated human embryonic stem cells. Samples were incubated in Brachyury polyclonal antibody (Product # PA5-46984) using a dilution of 10 µg/mL for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Brachyury in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm. Samples were incubated in Brachyury polyclonal antibody (Product # PA5-46984) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Brachyury in immersion fixed frozen sections of embryonic mouse notochord (E9.5). Samples were incubated in Brachyury polyclonal antibody (Product # PA5-46984) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assay of Brachyury in Mesoderm-differentiated BG01V human embryonic stem cells. Samples were immunoprecipitated with Brachyury polyclonal antibody (Product # PA5-46984) for 15 minutes in an ultrasonic bath using a dilution of 5 μg followed by a Biotinylated Anti-Goat IgG secondary antibody. Cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The VEGF promoter was detected by standard PCR.
