Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-9770-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Brachyury Monoclonal Antibody (X1AO2), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody X1AO2 recognizes human Brachyury, a 47 kDa protein encoded by the T gene and functioning as a nuclear transcription factor. This transcription factor binds to specific DNA regions near the palindromic sequence TCACACCT (T-site) through a region in its N-terminus, called the T-box. Brachyury functions during embryogenesis to regulate midline development by establishing the anterior/posterior axis through the regulation of genes involved in mesoderm formation and differentiation. Brachyury is the primary mesoderm marker used to distinguish differentiation of embryonic stem cells into a mesenchymal stem cell lineage. Variations in the T gene in humans are associated with neural tube defects and chordomas. Expression of brachyury is also increased in advanced stage lung tumors and tumor cell lines.
- Antibody clone number
- X1AO2
- Concentration
- 0.5 mg/mL
Submitted references The embryonic transcription factor Brachyury blocks cell cycle progression and mediates tumor resistance to conventional antitumor therapies.
Brachyury, a driver of the epithelial-mesenchymal transition, is overexpressed in human lung tumors: an opportunity for novel interventions against lung cancer.
The T-box transcription factor Brachyury promotes epithelial-mesenchymal transition in human tumor cells.
Huang B, Cohen JR, Fernando RI, Hamilton DH, Litzinger MT, Hodge JW, Palena C
Cell death & disease 2013 Jun 20;4:e682
Cell death & disease 2013 Jun 20;4:e682
Brachyury, a driver of the epithelial-mesenchymal transition, is overexpressed in human lung tumors: an opportunity for novel interventions against lung cancer.
Roselli M, Fernando RI, Guadagni F, Spila A, Alessandroni J, Palmirotta R, Costarelli L, Litzinger M, Hamilton D, Huang B, Tucker J, Tsang KY, Schlom J, Palena C
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jul 15;18(14):3868-79
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jul 15;18(14):3868-79
The T-box transcription factor Brachyury promotes epithelial-mesenchymal transition in human tumor cells.
Fernando RI, Litzinger M, Trono P, Hamilton DH, Schlom J, Palena C
The Journal of clinical investigation 2010 Feb;120(2):533-44
The Journal of clinical investigation 2010 Feb;120(2):533-44
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized HeLa cells using 5 µg/mL of Anti-Human Brachyury Purified followed by 10 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI; colocalization appears pink.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized HeLa cells using 5 µg/mL of Anti-Human Brachyury Purified followed by 10 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI; colocalization appears pink.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human testes tissue using 5 µg/mL of Anti-Human Brachyury Purified followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
