14-9770-80

antibody from Invitrogen Antibodies

Targeting: TBXT T

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

14-9770-80

Provider

Invitrogen Antibodies

Product name

Brachyury Monoclonal Antibody (X1AO2), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody X1AO2 recognizes human Brachyury, a 47 kDa protein encoded by the T gene and functioning as a nuclear transcription factor. This transcription factor binds to specific DNA regions near the palindromic sequence TCACACCT (T-site) through a region in its N-terminus, called the T-box. Brachyury functions during embryogenesis to regulate midline development by establishing the anterior/posterior axis through the regulation of genes involved in mesoderm formation and differentiation. Brachyury is the primary mesoderm marker used to distinguish differentiation of embryonic stem cells into a mesenchymal stem cell lineage. Variations in the T gene in humans are associated with neural tube defects and chordomas. Expression of brachyury is also increased in advanced stage lung tumors and tumor cell lines. Applications Reported: This X1AO2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This X1AO2 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This X1AO2 antibody has also been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

X1AO2

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

The embryonic transcription factor Brachyury blocks cell cycle progression and mediates tumor resistance to conventional antitumor therapies.

Huang B, Cohen JR, Fernando RI, Hamilton DH, Litzinger MT, Hodge JW, Palena C
Cell death & disease 2013 Jun 20;4:e682

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Brachyury, a driver of the epithelial-mesenchymal transition, is overexpressed in human lung tumors: an opportunity for novel interventions against lung cancer.

Roselli M, Fernando RI, Guadagni F, Spila A, Alessandroni J, Palmirotta R, Costarelli L, Litzinger M, Hamilton D, Huang B, Tucker J, Tsang KY, Schlom J, Palena C
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jul 15;18(14):3868-79

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The T-box transcription factor Brachyury promotes epithelial-mesenchymal transition in human tumor cells.

Fernando RI, Litzinger M, Trono P, Hamilton DH, Schlom J, Palena C
The Journal of clinical investigation 2010 Feb;120(2):533-44

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of fixed and permeabilized HeLa cells using 5 µg/mL of Anti-Human Brachyury Purified followed by 10 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI; colocalization appears pink.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of fixed and permeabilized HeLa cells using 5 µg/mL of Anti-Human Brachyury Purified followed by 10 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI; colocalization appears pink.