14-9770-80

TBXT antibody from Invitrogen Antibodies
T
Western blot
Recommended by provider
Immunocytochemistry
Supportive data in Antibodypedia
Immunohistochemistry
Supportive data in Antibodypedia

Antibody data

Product number
14-9770-80
Provider
Invitrogen Antibodies
Product name
Anti-Brachyury Monoclonal Antibody (X1AO2), eBioscience™
Provider product page
Invitrogen Antibodies - 14-9770-80
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody X1AO2 recognizes human Brachyury, a 47 kDa protein encoded by the T gene and functioning as a nuclear transcription factor. This transcription factor binds to specific DNA regions near the palindromic sequence TCACACCT (T-site) through a region in its N-terminus, called the T-box. Brachyury functions during embryogenesis to regulate midline development by establishing the anterior/posterior axis through the regulation of genes involved in mesoderm formation and differentiation. Brachyury is the primary mesoderm marker used to distinguish differentiation of embryonic stem cells into a mesenchymal stem cell lineage. Variations in the T gene in humans are associated with neural tube defects and chordomas. Expression of brachyury is also increased in advanced stage lung tumors and tumor cell lines. Applications Reported: This X1AO2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This X1AO2 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This X1AO2 antibody has also been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
X1AO2
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
Provider Type Product Number
- No reagents -